DNA Sequencing


DNA Sequencing


Benefits of our sequencing service
 
  • Comprehensive Microbiome Analysis: Sequencing provides a complete understanding of gut microbiome composition, including unculturable and previously unknown microbes.
  • Improved Pathogen Detection: It enhances the sensitivity of detecting foodborne pathogens, including non-culturable and emerging pathogens.
  • Species-Level Identification: Whole genomic DNA sequencing allows for precise identification of microbes at the species level, aiding in association studies with human diseases.
  • Understanding Disease Mechanisms: Sequencing improves insights into how the microbiome influences health and diseases, such as type 2 diabetes and gastrointestinal cancer.
  • Personalized Medicine: Insights from microbiome sequencing support the development of tailored healthcare strategies for individuals.
  • Biomarker Identification: Metagenomic sequencing can help identify potential biomarkers based on gut microbiome profiles, assisting in disease detection and monitoring.
  • Bespoke design: We offer dervices tailored to meet you needs, workflows and outputs designed to deliver to your requirements. 
What we offer
 
  • Sample Preparation: Our skilled technicians employ cutting-edge protocols to efficiently extract and purify high-quality genomic DNA from diverse microbial samples, including environmental, food, and clinical specimens, ensuring optimal preservation of microbial community structure while minimizing contamination to facilitate accurate sequencing and comprehensive metagenomic analysis.
  • Library preparation: Our expert team utilizes advanced library preparation protocols to construct high-quality genomic libraries from diverse microbial samples, employing optimized fragmentation techniques, precise size selection, and efficient adapter ligation to ensure comprehensive coverage and minimal bias, thereby enabling accurate and reproducible sequencing results for in-depth metagenomic analysis
  • Short-Read and Long-Read Sequencing: Whether you are sequencing amplicons, plasmids, bacteria, yeast or  phage our piplines can deilver projects in the areas of whole genome sequencing, gene panel testing, transcriptomics, de novo assembly, structural variant detection and metagenomics
Background
 
Welcome to our Genomic DNA Sequencing Service, an innovative solution that seamlessly integrates Short-Read Sequencing and Long-Read Sequencing technologies. This comprehensive service ensures a thorough analysis of genomic DNA, providing crucial insights using a variety of applications.
 
Our Short-Read Sequencing service makes use of high-throughput Illumina platforms to generate short DNA fragments, typically ranging from 400 to 600 base pairs, resulting in paired end 150 bp reads. We have an established and robust library preparation method with minimal variation on GC content that utilises over 1500 Unique Dual Indexes to meet your sequencing needs. The same library preparation method can be utilised for the sequencing of amplicons, plasmids, bacteria, yeast and phage, as well as complex microbial communities. We also offer a service where we perform library preparation and pooling only, which allows for cost effective sequencing at your preferred sequencing service provider if required.
 
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Our Long-Read Sequencing service employs ONT technologies to produce longer DNA fragments, dependent upon the input material, this approach facilitates the detection of structural variations and phasing variants, and enables de novo assembly. Long-Read Sequencing provides an in-depth exploration of the genome, uncovering details that contribute to a nuanced understanding of genetic information. We also offer a combined short- and long-read sequencing service to deliver complete, high quality small genome assembly.

In addition to our sequencing services, we also offer a long-term Genomic Consultancy Service. This consulting service provides expert guidance on experimental design, data interpretation, and strategic planning, tailored to meet your specific research or clinical requirements. Our commitment is to ensure that your genomics projects align with the most effective and impactful strategies, providing sustained support for your long-term objectives.

Our methodology extends beyond the generation of sequencing data, incorporating a robust bioinformatics pipeline for data processing, alignment, and analysis. Variant annotation adds functional insights to identified variants, and the integration of both short and long read data ensures a comprehensive genomic analysis. The reporting and interpretation phase is designed to be user friendly and accessible, while providing detailed reports which incorporate data visualisations and interpretations. This approach offers valuable insights for researchers, clinicians, and genetic counsellors alike, empowering you to make informed decisions based on a holistic genomic perspective.

If you would like to find out more about our range of DNA sequencing services, then please download our digital brochure here or begin discussing your project needs with us through our contact us page.


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Facilities and Equipment

The Quadram Institute building contains a range of state-of-the-art facilities and equipment, including a clinical research facility and Level 3 laboratory (CL3). 

Bespoke Scientific Research

Including food science and innovation, gut health (inc. microbiome) and microbes in the food chain.

Bioanalysis Service (SCFA, LCFA, BA)

Specialising in polyphenol analysis, our advanced techniques ensure accurate identification in client samples, offering precise results through tailored processing and consultation.

Microscopy Services

Our microscopy services cater to diverse biomedical and food research needs. Access cutting-edge equipment, personalised training, and comprehensive support for image acquisition, processing, and analysis.

Clinical Research Facility

The Clinical Research Facility at the Quadram Institute offers advanced resources for health research, including clinical spaces and a DXA scanner, with QIB Extra providing access to external clients.

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